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recom binant human bmp4  (MedChemExpress)


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    Structured Review

    MedChemExpress recom binant human bmp4
    Recom Binant Human Bmp4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recom+binant+human+bmp4/BMP-4%2C+Human/pm41645751-129-52-63
    Average 95 stars, based on 8 article reviews
    recom binant human bmp4 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Staining:

    Article Title: POSTN + fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling.
    Article Snippet: .. To confirm that BMP4 promotes macrophage polarization toward the M2 phenotype, THP‐1 (2x106 cells) were seeded in 6‐well plates and differentiated into macrophages using 50 ng/ml PMA for 48 h. After stabilization, macro‐ phages were assigned to different groups and then either left unstimulated or stimulated for an additional 48 h with recom‐ binant human BMP4 (50 or 100 ng/ml; cat. no. HY‐P7007; MedChemExpress).Macrophages were stained with FITC anti‐human CD163 antibody (cat. no. 333617; 1:50; Biolegend Inc.) and FITC anti‐human CD206 antibody (cat. no. 321103; 1:50; Biolegend Inc.) at 4 ̊C for 15 min and then centrifugation 1,000 x g at 4 ̊C for 5 min. Pellets were resuspended in 1 ml PBS and analyzed the expression of M2 macrophage polarization biomarkers (CD163 and CD206) was then analyzed by Flow cytometry (NovoCyte 2040R; ACEA Biosciences) equipped with NovoExpress software (version 1.6.3; https://explore. agilent.com/ACEA‐joins‐Agilent). .. To further to confirm the role of Smad signaling pathway, 10 μM LDN193189 inhibitor (cat. no. HY‐12071; MedChemExpress) was added to each well for 48 h during the BMP4 stimulation.

    Centrifugation:

    Article Title: POSTN + fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling.
    Article Snippet: .. To confirm that BMP4 promotes macrophage polarization toward the M2 phenotype, THP‐1 (2x106 cells) were seeded in 6‐well plates and differentiated into macrophages using 50 ng/ml PMA for 48 h. After stabilization, macro‐ phages were assigned to different groups and then either left unstimulated or stimulated for an additional 48 h with recom‐ binant human BMP4 (50 or 100 ng/ml; cat. no. HY‐P7007; MedChemExpress).Macrophages were stained with FITC anti‐human CD163 antibody (cat. no. 333617; 1:50; Biolegend Inc.) and FITC anti‐human CD206 antibody (cat. no. 321103; 1:50; Biolegend Inc.) at 4 ̊C for 15 min and then centrifugation 1,000 x g at 4 ̊C for 5 min. Pellets were resuspended in 1 ml PBS and analyzed the expression of M2 macrophage polarization biomarkers (CD163 and CD206) was then analyzed by Flow cytometry (NovoCyte 2040R; ACEA Biosciences) equipped with NovoExpress software (version 1.6.3; https://explore. agilent.com/ACEA‐joins‐Agilent). .. To further to confirm the role of Smad signaling pathway, 10 μM LDN193189 inhibitor (cat. no. HY‐12071; MedChemExpress) was added to each well for 48 h during the BMP4 stimulation.

    Expressing:

    Article Title: POSTN + fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling.
    Article Snippet: .. To confirm that BMP4 promotes macrophage polarization toward the M2 phenotype, THP‐1 (2x106 cells) were seeded in 6‐well plates and differentiated into macrophages using 50 ng/ml PMA for 48 h. After stabilization, macro‐ phages were assigned to different groups and then either left unstimulated or stimulated for an additional 48 h with recom‐ binant human BMP4 (50 or 100 ng/ml; cat. no. HY‐P7007; MedChemExpress).Macrophages were stained with FITC anti‐human CD163 antibody (cat. no. 333617; 1:50; Biolegend Inc.) and FITC anti‐human CD206 antibody (cat. no. 321103; 1:50; Biolegend Inc.) at 4 ̊C for 15 min and then centrifugation 1,000 x g at 4 ̊C for 5 min. Pellets were resuspended in 1 ml PBS and analyzed the expression of M2 macrophage polarization biomarkers (CD163 and CD206) was then analyzed by Flow cytometry (NovoCyte 2040R; ACEA Biosciences) equipped with NovoExpress software (version 1.6.3; https://explore. agilent.com/ACEA‐joins‐Agilent). .. To further to confirm the role of Smad signaling pathway, 10 μM LDN193189 inhibitor (cat. no. HY‐12071; MedChemExpress) was added to each well for 48 h during the BMP4 stimulation.

    Flow Cytometry:

    Article Title: POSTN + fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling.
    Article Snippet: .. To confirm that BMP4 promotes macrophage polarization toward the M2 phenotype, THP‐1 (2x106 cells) were seeded in 6‐well plates and differentiated into macrophages using 50 ng/ml PMA for 48 h. After stabilization, macro‐ phages were assigned to different groups and then either left unstimulated or stimulated for an additional 48 h with recom‐ binant human BMP4 (50 or 100 ng/ml; cat. no. HY‐P7007; MedChemExpress).Macrophages were stained with FITC anti‐human CD163 antibody (cat. no. 333617; 1:50; Biolegend Inc.) and FITC anti‐human CD206 antibody (cat. no. 321103; 1:50; Biolegend Inc.) at 4 ̊C for 15 min and then centrifugation 1,000 x g at 4 ̊C for 5 min. Pellets were resuspended in 1 ml PBS and analyzed the expression of M2 macrophage polarization biomarkers (CD163 and CD206) was then analyzed by Flow cytometry (NovoCyte 2040R; ACEA Biosciences) equipped with NovoExpress software (version 1.6.3; https://explore. agilent.com/ACEA‐joins‐Agilent). .. To further to confirm the role of Smad signaling pathway, 10 μM LDN193189 inhibitor (cat. no. HY‐12071; MedChemExpress) was added to each well for 48 h during the BMP4 stimulation.

    Software:

    Article Title: POSTN + fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling.
    Article Snippet: .. To confirm that BMP4 promotes macrophage polarization toward the M2 phenotype, THP‐1 (2x106 cells) were seeded in 6‐well plates and differentiated into macrophages using 50 ng/ml PMA for 48 h. After stabilization, macro‐ phages were assigned to different groups and then either left unstimulated or stimulated for an additional 48 h with recom‐ binant human BMP4 (50 or 100 ng/ml; cat. no. HY‐P7007; MedChemExpress).Macrophages were stained with FITC anti‐human CD163 antibody (cat. no. 333617; 1:50; Biolegend Inc.) and FITC anti‐human CD206 antibody (cat. no. 321103; 1:50; Biolegend Inc.) at 4 ̊C for 15 min and then centrifugation 1,000 x g at 4 ̊C for 5 min. Pellets were resuspended in 1 ml PBS and analyzed the expression of M2 macrophage polarization biomarkers (CD163 and CD206) was then analyzed by Flow cytometry (NovoCyte 2040R; ACEA Biosciences) equipped with NovoExpress software (version 1.6.3; https://explore. agilent.com/ACEA‐joins‐Agilent). .. To further to confirm the role of Smad signaling pathway, 10 μM LDN193189 inhibitor (cat. no. HY‐12071; MedChemExpress) was added to each well for 48 h during the BMP4 stimulation.



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    95
    MedChemExpress recom binant human bmp4
    Recom Binant Human Bmp4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recom+binant+human+bmp4/BMP-4%2C+Human/pm41645751-129-52-63
    Average 95 stars, based on 1 article reviews
    recom binant human bmp4 - by Bioz Stars, 2026-11
    95/100 stars
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